Evaluation of Digital PCR for Absolute RNA Quantification
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چکیده
منابع مشابه
Evaluation of Digital PCR for Absolute RNA Quantification
Gene expression measurements detailing mRNA quantities are widely employed in molecular biology and are increasingly important in diagnostic fields. Reverse transcription (RT), necessary for generating complementary DNA, can be both inefficient and imprecise, but remains a quintessential RNA analysis tool using qPCR. This study developed a Transcriptomic Calibration Material and assessed the RT...
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when duplicate blots were probed with the avidin-peroxidase conjugate (Figure 1B, lanes 5′–8′) ExtrAvidin® (Sigma, St. Louis, MO, USA). Unlike the secondary antibody conjugate used in Figure 1A, the avidin-peroxidase conjugate used in Figure 1B did not recognize the electrophoresed immunoglobulins within the immunoprecipitate. Moreover, specific bands of ezrin were readily detected in immunopre...
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ژورنال
عنوان ژورنال: PLoS ONE
سال: 2013
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0075296